Adaptation of an Enzyme Immunoassay to Assess Urinary Cotinine in Nonsmokers Exposed to Tobacco Smoke

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Urinary thromboxane, prostacyclin, cortisol, and 8-hydroxy-2'-deoxyguanosine in nonsmokers exposed and not exposed to environmental tobacco smoke.

This study tested the hypotheses that (1) increased platelet aggregation, as measured by 2,3-dinor-thromboxane B(2) (Tx-M) and 2,3-dinor-6-keto-prostaglandin F(1alpha) (PGI-M), and (2) increased oxidative stress, as measured by 8-Hydroxy-2'-deoxyguanosine (8-OHdG), would occur in ETS-exposed nonsmokers as compared with non-ETS-exposed nonsmokers. The concentrations of the stable urinary metabol...

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Self-Reported Exposure to Second-Hand Smoke and Positive Urinary Cotinine in Pregnant Nonsmokers

PURPOSE This cross-sectional study aimed to examine the association between self-reported exposure status to second-hand smoke and urinary cotinine level in pregnant nonsmokers. MATERIALS AND METHODS We recruited pregnant nonsmokers from the prenatal care clinics of a university hospital and two community health centers, and their urinary cotinine concentrations were measured. RESULTS Among...

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Respiratory illness in nonsmokers chronically exposed to tobacco smoke in the work place.

We evaluated CO levels as an index of cigarette smoke in the work place and analyzed diary entries on respiratory symptoms, eye irritation, chest colds and lost days from work due to respiratory illness in 40 passive smokers (nonsmokers chronically exposed to tobacco smoke in the work place) and 40 control subjects (nonsmokers not exposed to tobacco smoke in the work place) matched for age and ...

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Combined SPE and HPTLC as a Screening Assay of Urinary Cotinine from Male Adolescents Exposed to Environmental Tobacco Smoke**

Cotinine, as the main metabolite of nicotine, has been determined in urine using solid-phase extraction and the high-performance thin-layer chromatographic (SPE-HPTLC) method. The urine samples were collected from a group of 35 male adolescents which were moderate or significantly exposed to home environmental tobacco smoke (ETS). l-methyl-2-pyrrolidinone was used as the internal standard in th...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 2001

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/47.5.950